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GeneSifter® Analysis Edition:
Small RNA Analysis using Next Generation Sequencing
In recent years non-coding small RNAs,
such as microRNAs (miRNA), and small
interfering RNAs (siRNA), have been
increasingly recognized as a significant
regulatory factor in many biological
processes. These short 20-30 nucleotide
sequences bind to their targets and either
inhibit translation or trigger destruction
of their mRNA targets.
Intuitive Reports
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Alignment statistics:
- Number of reads uniquely mapped to each reference.
- Known small RNAs
- Novel small RNAs
- Full accounting of all reads including the number of mismatches for mapped reads .
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Known small RNA list:
- Expression values for each identified small RNA.
- Total number of reads.
- Normalized counts: Reads per Million (RPM).
- Thumbnail of read coverage over small RNA, which is live-linked to an integrated viewer.
- Live-links to miRBase for more information including target prediction.
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Novel small RNA list:
- Expression values for each identified potentially novel small RNA.
- Total number of reads.
- Normalized counts: Reads per Million (RPM).
- Thumbnail of read cluster, that is live-linked to an integrated viewer allowing visualization of reads in genome space (shows genes, transcripts, and base variants present around the potentially novel small RNA).
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Filter List:
- Expression values for filtered references.
- Total number of reads.
- Normalized counts: Reads per Million (RPM).
- Thumbnail map of read coverage live-lined to integrated viewer.
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Contact us for more information or to find out how Geospiza can help with your analysis or lab automation. 877.Web.Gene or info@geospiza.com or Request More Info |
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